| Purified GST-tagged human RXR-gamma LBD |
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Retinoid X receptor gamma (RXR-gamma or RXRG) is also known as NR2B3 (nuclear receptor subfamily 2, group B, member 3). GST-tagged RXR-gamma ligand-binding domain (RXRG-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 14 a.a. flexible linker and a 247 a.a. RXRG-LBD, Acc# NP_008848.1, amino acids 217-463 (details). GST tagged RXRG-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). RXRG-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies. |
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Storage buffer 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, 3 mM Dithiothreitol, 7 mM reduced glutathione, 20% glycerol. Purity >90% Applications Ligand binding and coactivator peptide recruitment assays. Handling upon arrival Immediately store at -80°C. Avoid freeze-thaw cycles. While working, please keep sample on ice. |
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Fig. 1. SDS-PAGE analysis of purified RXR-gamma LBD. Proteins were separated in 4-20% SDS-PAGE and stained with Coomassie blue. 1. RXRG-LBD. 2. Low range molecular weight marker (Bio-Rad). |