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AB Vector, founded in 2001, is a leader in the design and development of novel vectors facilitating protein folding. We are the only suppliers of a higher eukaryotic expression system synthesizing ensembles of molecular chaperones at the levels capable of assisting the proper folding of abundantly expressed foreign proteins. Alan Schwartz, PhD, MBA, joined the company in 2003. He is responsible for licensing for AB Vector. Dr. Schwartz, a medicinal chemist by training, worked at Hoffmann-LaRoche for 14 years in a variety of pharmaceutical R&D positions. For the past 5 years, he has been representing the commercial interests of Sweden's Karolinska Institute through their research technology company, Karolinska Innovations AB. Peter L. Pingerelli, Ph.D. joined the company in 2004 to organize and promote the commercialization of these novel reagents. Dr. Pingerelli is a protein chemist and NMR spectroscopist by training. He has been a senior manager at research reagent companies and has a 14-year successful track record in business development, new product development, sales and marketing, and management in the Biotech industry. |
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Mission
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Our mission is to provide innovative tools to facilitate the production of correctly folded proteins. We plan to serve a vital role in helping the scientific community to
understand protein structure and function. To achieve that mission, our vision is to extend our technological leadership position to cover most of the aspects of protein
folding in major cell compartments.
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Advantages
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Up to now, developments in the protein expression have been largely focused on improvements in generating recombinant clones and increasing the levels of protein production.
We recently developed ProFold,™ patent pending, the first commercially available vectors facilitating protein folding. The main advantage of ProFold™ vectors is the overexpression of molecular chaperones in either the
cytoplasm or the ER, which help to fold the protein of interest. We have tested our vectors with mammalian, yeast and plant proteins and observed significant improvement
in yeild of biologically active proteins compared to the same proteins expressed using conventional baculovirus vectors. We have always observed large improvements in the solubility of proteins previously
observed to be completely insoluble when expressed in E.coli using ProFold™ vectors.
Another advantage of ProFold™ vectors is a convenience of optimization of your protein expression system via green fluorescense. ProFold™ vectors provide
for expression of Aequorea victoria green fluorescent protein (GFP). This allows monitoring of virus propagation, optimization of cultivation conditions, and titrating virus stocks by "terminal green dilution". It saves time and effort; no need for expensive baculovirus titration kits or specialized equipment. The only essential pieces required are the same 265 nm UV transilluminator and digital camera routinely used for ethidium bromide stained gels. In addition, you can count green foci of infected cells using fluorescence microscope or take advantage of the flow cytometry
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