Glycoproteins
	Calreticulin is a major molecular chaperone participating in glycoprotein folding. Improved folding of glycoproteins can be achieved using our ProFold™-ER1 vector. ProFold™-ER1 provides for a high level of expression for both the target glycoprotein and human calreticulin using the same recombinant baculovirus genome. It can be combined with any of our plasmid transfer vectors. Since most of the glycoproteins are either secreted or transmembrane proteins, you can find detailed recommendations in Secreted proteins or Transmembrane proteins. A smaller group of glycoproteins are resident proteins of the endoplasmic reticulum. Recommendations for expression of secreted proteins are largely applicable to the latter group.
	Relevant products
	Plasmid transfer vectors                             Click on the product name for technical information! Cat # Product Specification Unit size Price* B1 pVL1393 High level of target protein expression 50 μl, 0.5 μg/μl $340.00 B3 pAB-bee Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER Recommended for high level expression of secreted and transmembrane proteins 50 μl, 0.5 μg/μl $495.00 B3H pAB-bee™-8xHis Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER Recommended for high level expression of secreted and transmembrane proteins N- or C-terminal 8xHis tag for affinity purification 50 μl, 0.5 μg/μl $495.00 B7 pAB-6xHis-MBP™ High level expression of proteins with cleavable N-terminal 6xHis MBP tag High level expression of proteins with C-terminal MBP tag MBP tag improves protein solubility and yield 50 μl, 0.5 μg/μl $495.00   Linearized baculovirus vector DNA CsCl purified and digested with Bsu 36.I                  Click on the product name for technical information! Cat # Product Specification Unit size Price* A4 ProFold™-ER1 Overexpressed calreticulin facilitates glycoprotein folding in the ER Overexpressed PDI facilitates folding of cysteine-rich proteins GFP marker to monitor virus propagation High level of target protein expression 25 μl, 2.5 μg 5 transfections $830.00   Controls                                                                    Click on the product name for technical information! Cat # Product Specification Unit size Price* C13 NC (negative control) Recombinant baculovirus stock Extracts of cells infected with NC is an ideal negative control as there is no      polyhedrin or target protein expression NC extracts are run side by side in SDS-PAGE with extracts of cells infected with      recombinant baculoviruses Recombinant protein is detected as a prominent band which is absent in NC      extracts 1 ml, 108 p.f.u./ml $195.00 C14 GC (green control) Recombinant baculovirus stock Positive control baculovirus in experiments where GFP fluorescence is used to      monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks 1 ml, 108 p.f.u./ml $195.00 C15 GCP (green control plasmid) Plasmid transfer vector DNA Positive control for transfection with linearized GFP- baculovirus DNA. Green      fluorescence of cells is indicative of successful transfection. 50 μl, 0.2 μg/μl $145.00
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	If you have not worked with baculovirus expression system, also referred to as baculovirus expression vector system or BEVS, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
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