|Rules to achieve about as high recombinant protein expression levels using baculoviruses as in E.coli.
Maintenance of insect cells
Requirements to monolayers
Baculovirus stock propagation
|Gently mix 0.1 μg of plasmid DNA
containing a gene of interest, 5 μl of
baculovirus DNA and H20 up to 50 μl.
| Add dropwise 50 μl
of 10% Profectin™
to the DNA mixture
from step 1.
| Add dropwise DNA-Profectin™ emulsion from
step 2 to semi-confluent monolayer of insect
cells. Incubate for 60-72 h, harvest recombinant
baculovirus and study protein expression.