AB Vector - Baculovirus tutorial

                                                              Baculovirus tutorial
                         A set of rules to achieve about as high recombinant protein expression levels using baculoviruses as in E.coli.
            
 
Gently mix 0.1 μg of plasmid DNA
containing a gene of interest, 5 μl of
baculovirus DNA and H20 up to 50 μl.		      Add dropwise 50 μl
     of 10% Profectin™
     to the DNA mixture
     from step 1.		      Add dropwise DNA-Profectin™ emulsion from
     step 2 to semi-confluent monolayer of insect
     cells. Incubate for 60-72 h, harvest recombinant
     baculovirus and study protein expression.

Home        About us        Technology        Products        Order        Pricing        Licensing        Contact us        Custom service
2003 - 2009 © AB Vector LLC. All rights reserved.