His tag vectors
	The polyhistidine tag is one of the most widely used tags. It can be provided on the N- or C-terminus of target proteins and can be used for protein purification by immobilized metal affinity chromatography and for immunodetection. Target proteins can be provided with a cleavable N-terminal 6xHis tag using our pAB-6xHis™ vector, or with a C-terminal FLAG-8xHis tag using pVL-FH vector. The pAB-6xHis-MBP™ vector provides a cleavable N-terminal 6xHis-MBP tag. MBP (maltose binding protein) moiety is well documented to increase yield and solubility of target fusion proteins. Furthermore, it ensures exposure of the 6xHis tag on the surface of the fusion protein, which facilitates affinity purification under native conditions. Another vector, pAB-bee™-8xHis is ideal for the expression of polyhistidine-tagged proteins which are translocated into the endoplasmic reticulum (ER), such as secreted and transmembrane proteins. Using this vector, the target protein can be provided with a strong signal sequence to ensure its efficient translocation into the ER, followed by a signal peptidase cleavage site and an N-terminal 8xHis tag. Optionally the 8xHis tag can be also provided on the C-terminus of a target protein. A similar pAB-bee vector encodes a strong signal sequence and an 8xHis tag which is provided on the C- terminus of the translocated proteins, but it does not encode for the N-terminal tag. A derivative of this vector pAB-bee™-FH encodes strong signal sequence and C-terminal dual FLAG-8xHis tag. The plasmid transfer vectors described above can be combined with any of the listed below linearized baculovirus DNA vectors, i.e. ProEasy™, ProGreen™, ProFold™-ER1, ProFold™-C2, ProFold™-PDI, and ProFold™-0. Click on TECHNOLOGY for more information on baculovirus expression system. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
	Relevant products
	Plasmid transfer vectors                             Click on the product name for technical information! Cat # Product Specification Unit size Price* B3 pAB-bee Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER Recommended for high level expression of secreted and transmembrane proteins 50 μl, 0.5 μg/μl $495.00 B3H pAB-bee™-8xHis Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER Recommended for high level expression of secreted and transmembrane proteins N- or C-terminal 8xHis tag for affinity purification 50 μl, 0.5 μg/μl $495.00 B3FH pAB-bee™-FH Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER Recommended for high level expression of secreted and transmembrane proteins C-terminal FLAG-8xHis tag for affinity purification and immonodetection 50 μl, 0.5 μg/μl $495.00 B4 pAB-6xHis™ High level expression of proteins with cleavable N-terminal 6xHis tag 50 μl, 0.5 μg/μl $295.00 B7 pAB-6xHis-MBP™ High level expression of proteins with cleavable N-terminal 6xHis MBP tag High level expression of proteins with C-terminal MBP tag MBP tag improves protein solubility and yield 50 μl, 0.5 μg/μl $495.00 B9 pVL-FH High level expression of proteins with C-terminal FLAG-8xHis tag 50 μl, 0.5 μg/μl $295.00   Linearized baculovirus vector DNA CsCl purified and digested with Bsu 36.I                  Click on the product name for technical information! Cat # Product Specification Unit size Price* A1 ProGreen™ High level of target protein expression GFP marker to monitor virus propagation 25 μl, 2.5 μg 5 transfections $199.00 A3 ProFold™-C2 Overexpressed Hsp40 and Hsc70 chaperones facilitate target protein folding in the      cytoplasm GFP marker to monitor virus propagation Slightly reduced level of protein expression 25 μl, 2.5 μg 5 transfections $695.00 A4 ProFold™-ER1 Overexpressed calreticulin facilitates glycoprotein folding in the ER Overexpressed PDI facilitates folding of cysteine-rich proteins GFP marker to monitor virus propagation High level of target protein expression 25 μl, 2.5 μg 5 transfections $695.00 A7 ProFold™-PDI Overexpressed PDI facilitates folding of cysteine-rich proteins High level of target protein expression from the same genome as PDI 25 μl, 2.5 μg 5 transfections $465.00 A9 ProFold™-0 The best value baculovirus vector High level of target protein expression 25 μl, 2.5 μg 5 transfections $195.00 A10S ProEasy™ The easiest generation of recombinant baculoviruses Ideal for HTP applications High level of target protein expression 25 μl, 2.5 μg 5 transfections $599.00   Controls                                                                    Click on the product name for technical information! Cat # Product Specification Unit size Price* C13 NC (negative control) Recombinant baculovirus stock Extracts of cells infected with NC is an ideal negative control as there is no      polyhedrin or target protein expression NC extracts are run side by side in SDS-PAGE with extracts of cells infected with      recombinant baculoviruses Recombinant protein is detected as a prominent band which is absent in NC      extracts 1 ml, 108 p.f.u./ml $195.00 C14 GC (green control) Recombinant baculovirus stock Positive control baculovirus in experiments where GFP fluorescence is used to      monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks 1 ml, 108 p.f.u./ml $195.00 C15 GCP (green control plasmid) Plasmid transfer vector DNA Positive control for transfection with linearized GFP- baculovirus DNA. Green      fluorescence of cells is indicative of successful transfection. 50 μl, 0.2 μg/μl $145.00
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	If you have not worked with recombinant baculoviruses, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
	Click on PRODUCTS to view the entire list of recombinant baculovirus-related products.

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