| pAB-GST™ | Cat. #B5 | |
| DNA Sequence | ||
| pAB-GST™ plasmid transfer vector is designed for expression of proteins with N-terminal glutathione S-transferase (GST) tag (Smith D.B. & Johnson K.S., Gene, 15:31-40, 1988). A PCR fragment encoding a protein of interest can be cloned in lieu of the polyhedrin promoter and GST tag using any of the unique restriction endonuclease sites (BamHI, EcoRI, SmaI, NotI, PstI, BglII) located in the MCS. Proteins are expressed under control of strong very late polyhedrin promoter in the polyhedrin site of baculovirus DNA. Affinity purification of the expressed proteins can be achieved using Glutathione Sepharose™ 4B resin (Amersham Biosciences). If desired, GST tag can be removed with PreScission™ protease which cleaves at specific site (LEVLFQGPL) encoded at nucleotides 4798-4824 of the vector. | ||
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| Sepharose and PreScission are trademarks of Amersham Biosciences. If you have not worked with baculovirus expression system, also referred to as baculovirus expression vector system or BEVS, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies. Click on PRODUCTS to view the entire list of recombinant baculovirus-related products. |
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