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Overexpressed cytoplasmic proteins are often misfolded which results in the accumulation of biologically inactive aggregates (inclusion bodies). In unmodified cells these proteins do not aggregate since they are expressed at low yield (typically below 0.1% of the total cell protein). At such low concentrations molecular chaperones are in excess to the newly synthesized proteins which ensures their correct folding. However, if a protein is overexpressed, its concentration by far exceeds concentration of molecular chaperones in the cytoplasm, resulting in target protein misfolding and aggregation.
ProFold™-C1 and ProFold™-C2 linearized baculovirus vector DNA can provide for overexpression of both a cytoplasmic protein of interest and major cytoplasmic molecular chaperones Hsp40 and Hsc70. This often improves the yield of correctly folded cytoplasmic target proteins as compared to the same target proteins expressed using conventional vectors (Bac-to-Bac™**, BaculoGold™***, and BacPAK6™****) which do not provide molecular chaperones. Click on TECHNOLOGY for the details.
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Use pVL1393 plasmid transfer vector if you wish to express target proteins without affinity tags.
Use pAB-6xHis™ plasmid transfer vector if you wish to express target proteins with a polyhistidine tag.
Use pAB-GST™ plasmid transfer vector if you wish to express target proteins with a GST tag.
Use pAB-MBP™ plasmid transfer vector if you wish to express target proteins with a maltose binding protein (MBP) tag. Note that an MBP tag could improve protein solubility.
Combine any of the above plasmid transfer vectors with ProFold™-C2 linearized baculovirus DNA if you wish to provide Hsp40 and Hsc70 molecular chaperones.
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The above features can be mixed and matched in various combinations, while supporting a high level of target protein expression. They can also be combined with "plain vectors" which only provide a high level of protein expression (pVL1393, ProEasy™, ProGreen™, ProFold™-0). A plasmid transfer vector is always combined with a baculovirus DNA vector, and some combinations are available as Kits. Click on MATERIALS AND METHODS for details. |
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1.Solubility of a cytoplasmic protein is a problem. Insert gene encoding target cytoplasmic protein in pVL1393 plasmid vector if no affinity tag is required. Alternatively, use the pAB-GST™, pAB-6xHis™, or pAB-MBP™ vectors to provide target proteins with GST, His, or MBP tags. Co-transfect plasmid construct with ProFold™-C2 linearized baculovirus DNA.
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2.Target cytoplasmic protein is not problematic. Use ProEasy™ linearized baculovirus DNA, as it is the easiest method to construct recombinant baculoviruses. It is compatible with any of the pVL1393, pAB-GST™, pAB-6xHis™, or pAB-MBP™ plasmid transfer vectors.
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3. You have little experience with recombinant baculoviruses. Get a quick update on relevant recombinant baculovirus technologies at Materials and methods. Use ProEasy™ linearized baculovirus DNA, as it is the easiest method to construct recombinant baculoviruses. It is compatible with any of the pVL1393, pAB-GST™, pAB-6xHis™, or pAB-MBP™ plasmid transfer vectors.
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Plasmid transfer vectors
Click on the product name for technical information!
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| B1 |
pVL1393 |
 High level of target protein expression |
50 μl, 0.5 μg/μl |
$295.00
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| B4 |
pAB-6xHis™ |
High level expression of proteins with cleavable N-terminal 6xHis tag
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50 μl, 0.5 μg/μl |
$295.00
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| B5 |
pAB-GST™ |
High level expression of proteins with cleavable N-terminal GST tag |
50 μl, 0.5 μg/μl |
$295.00
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| B6 |
pAB-MBP™ |
High level expression of proteins with cleavable N-terminal MBP tag
MBP tag improves protein solubility and yield |
50 μl, 0.5 μg/μl |
$395.00
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| B7 |
pAB-6xHis-MBP™ |
High level expression of proteins with cleavable N-terminal 6xHis MBP tag
High level expression of proteins with C-terminal MBP tag
MBP tag improves protein solubility and yield |
50 μl, 0.5 μg/μl |
$495.00
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Linearized baculovirus vector DNA
CsCl purified and digested with Bsu 36.I
Click on the product name for technical information!
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| A1 |
ProGreen™ |
High level of target protein expression
GFP marker to monitor virus propagation |
25 μl, 2.5 μg
5 transfections |
$199.00
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| A3 |
ProFold™-C2 |
Overexpressed Hsp40 and Hsc70 chaperones facilitate target protein folding in the cytoplasm
GFP marker to monitor virus propagation
Slightly reduced level of protein expression |
25 μl, 2.5 μg
5 transfections |
$695.00
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| A9 |
ProFold™-0 |
The best value baculovirus vector
High level of target protein expression
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25 μl, 2.5 μg
5 transfections |
$195.00
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| A10S |
ProEasy™ |
The easiest generation of recombinant baculoviruses
Ideal for HST applications
High level of target protein expression |
25 μl, 2.5 μg
5 transfections |
$599.00
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Controls
Click on the product name for technical information!
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| C13 |
NC (negative control) Recombinant baculovirus stock |
Extracts of cells infected with NC is an ideal negative control as there is no polyhedrin or target protein expression
NC extracts are run side by side in SDS-PAGE with extracts of cells infected with recombinant baculoviruses
Recombinant protein is detected as a prominent band which is absent in NC extracts |
1 ml, 108 p.f.u./ml |
$195.00
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| C14 |
GC (green control) Recombinant baculovirus stock |
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks |
1 ml, 108 p.f.u./ml |
$295.00
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| C15 |
GCP (green control plasmid) Plasmid transfer vector DNA |
Positive control for transfection with linearized GFP- baculovirus DNA. Green fluorescence of cells is indicative of successful transfection.
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50 μl, 0.2 μg/μl |
$145.00
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*Pricing and online ordering is for US and Canadian customers only. Contact Tel: 1-866-683-2867, Fax 1-760-454-2465, email info@abvector.com
to order in North America. Go to PRODUCTS page to order on line. For international ordering go to Order page or Home page.
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If you have not worked with recombinant baculoviruses, you can get a quick update at MATERIALS AND METHODS. |
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Click on PRODUCTS to view the entire list of recombinant baculovirus-related products. |