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The GST (glutathione S-transferase) tag is one of the most widely used tags for protein affinity purification and immunodetection. Target proteins can be provided with a cleavable N-terminal GST tag using our pAB-GST™ vector.
The pAB-GST™ plasmid transfer vector described above can be combined with any of the listed below linearized baculovirus DNA vectors, i.e. ProEasy™, ProGreen™, ProFold™-ER1, ProFold™-C2, ProFold™-PDI, and ProFold™-0. Click on TECHNOLOGY for more information on baculovirus expression system. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies for details.
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Plasmid transfer vectors
Click on the product name for technical information!
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| B5 |
pAB-GST™ |
 High level expression of proteins with cleavable N-terminal GST tag |
50 μl, 0.5 μg/μl |
$295.00
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Linearized baculovirus vector DNA
CsCl purified and digested with Bsu 36.I
Click on the product name for technical information!
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| A1 |
ProGreen™ |
High level of target protein expression
GFP marker to monitor virus propagation |
25 μl, 2.5 μg
5 transfections |
$199.00
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| A3 |
ProFold™-C2 |
Overexpressed Hsp40 and Hsc70 chaperones facilitate target protein folding in the cytoplasm
GFP marker to monitor virus propagation
Slightly reduced level of protein expression |
25 μl, 2.5 μg
5 transfections |
$695.00
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| A4 |
ProFold™-ER1 |
Overexpressed calreticulin facilitates glycoprotein folding in the ER
Overexpressed PDI facilitates folding of cysteine-rich proteins
GFP marker to monitor virus propagation
High level of target protein expression |
25 μl, 2.5 μg
5 transfections |
$695.00
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| A7 |
ProFold™-PDI |
Overexpressed PDI facilitates folding of cysteine-rich proteins
High level of target protein expression from the same genome as PDI |
25 μl, 2.5 μg
5 transfections |
$465.00
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| A9 |
ProFold™-0 |
The best value baculovirus vector
High level of target protein expression
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25 μl, 2.5 μg
5 transfections |
$195.00
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| A10S |
ProEasy™ |
The easiest generation of recombinant baculoviruses
Ideal for HTP applications
High level of target protein expression |
25 μl, 2.5 μg
5 transfections |
$599.00
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Controls
Click on the product name for technical information!
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| C13 |
NC (negative control) Recombinant baculovirus stock |
Extracts of cells infected with NC is an ideal negative control as there is no polyhedrin or target protein expression
NC extracts are run side by side in SDS-PAGE with extracts of cells infected with recombinant baculoviruses
Recombinant protein is detected as a prominent band which is absent in NC extracts |
1 ml, 108 p.f.u./ml |
$195.00
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| C14 |
GC (green control) Recombinant baculovirus stock |
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks |
1 ml, 108 p.f.u./ml |
$195.00
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| C15 |
GCP (green control plasmid) Plasmid transfer vector DNA |
Positive control for transfection with linearized GFP- baculovirus DNA. Green fluorescence of cells is indicative of successful transfection.
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50 μl, 0.2 μg/μl |
$145.00
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*Pricing and online ordering is for US and Canadian customers only. Contact Tel: 1-866-683-2867, Fax 1-760-454-2465, email info@abvector.com
to order in North America. Go to PRODUCTS page to order on line. For international ordering go to Order page or Home page.
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If you have not worked with recombinant baculoviruses, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies. |
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Click on PRODUCTS to view the entire list of recombinant baculovirus-related products. |