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Our pVL-GFP vector allows expression of target proteins in-fusion with the C-terminal GFP tag. The GFP tag is widely used for visualization and isolation of protein complexes, studies of protein-protein interactions, protein trafficking within the cells, and mechanisms of protein degradation (Cristea et al., Mol Cell Proteomics, 4:1933-41, 2005; Michaelson and Philips, Methods Enzymol, 406:296-315, 2006; Hayes et al., Cancer Lett., 206:129-35, 2004 ; Takeuchi et al., EMBO J., 26:123-31, 2007).
Especially useful is the ability of the GFP tag to misfold and loose its fluorescence if its fusion partner is misfolded. This property was used for selection of target protein mutants with improved solubility (Van den Berg et at, J Biotechnol, 121:291-8, 2006), for finding soluble proteins derived from randomly fragmented cDNAs (Nakayama and Ohara, Biochem Biophys Res Commun, 312:825-30, 2003), and in studies of protein aggregation (Schrodel and de Marco, BMC Biochem, 6:10, 2005).
Of particular interest are studies of GFP fusion aggregates with proteins related to protein folding diseases (Krobitsch and Lindquist, Proc Natl Acad Sci U S A, 97:1589-94, 2000; Wang et al., Hum Mol Genet., 14:3673-84, 2005; Pandey et al., Exp Neurol., 197:515-20, 2006; Jiang et al., J Biol Chem., 282:7912-20, 2007). Such fusions could be used for high throughput screening of compounds that inhibit aggregation of these important drug targets (Gazit, ASC Chemical Biology, 1:417-19, 2006). AB Vector offers some protein folding diseases targets in-fusion with GFP.
The pVL-GFP plasmid transfer vector can be combined with any of the listed below linearized baculovirus DNA vectors that do not encode GFP, i.e. ProEasy™, ProFold™-PDI, and ProFold™-0. Click on TECHNOLOGY for more information on baculovirus expression system. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
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Plasmid transfer vectors
Click on the product name for technical information!
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| B8 |
pVL-GFP |
 High level expression of proteins with C-terminal GFP tag |
50 μl, 0.5 μg/μl |
$395.00
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Linearized baculovirus vector DNA
CsCl purified and digested with Bsu 36.I
Click on the product name for technical information!
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| A7 |
ProFold™-PDI |
Overexpressed PDI facilitates folding of cysteine-rich proteins
High level of target protein expression from the same genome as PDI |
25 μl, 2.5 μg
5 transfections |
$465.00
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| A9 |
ProFold™-0 |
The best value baculovirus vector
High level of target protein expression
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25 μl, 2.5 μg
5 transfections |
$195.00
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| A10S |
ProEasy™ |
The easiest generation of recombinant baculoviruses
Ideal for HTP applications
High level of target protein expression |
25 μl, 2.5 μg
5 transfections |
$599.00
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Controls
Click on the product name for technical information!
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| C13 |
NC (negative control) Recombinant baculovirus stock |
Extracts of cells infected with NC is an ideal negative control as there is no polyhedrin or target protein expression
NC extracts are run side by side in SDS-PAGE with extracts of cells infected with recombinant baculoviruses
Recombinant protein is detected as a prominent band which is absent in NC extracts |
1 ml, 108 p.f.u./ml |
$195.00
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| C14 |
GC (green control) Recombinant baculovirus stock |
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks |
1 ml, 108 p.f.u./ml |
$195.00
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| C15 |
GCP (green control plasmid) Plasmid transfer vector DNA |
Positive control for transfection with linearized GFP- baculovirus DNA. Green fluorescence of cells is indicative of successful transfection.
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50 μl, 0.2 μg/μl |
$145.00
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Recombinant baculovirus stocks for expression of protein folding diseases targets in-fusion with GFP
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| PD21 |
BV-Syn-GFP
Recombinant baculovirus stock |
Expression of alpha synuclein-GFP fusion protein |
1 ml, 108 p.f.u./ml |
$499.00
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| PD29 |
BV-SOD1-GFP
Recombinant baculovirus stock |
Expression of SOD1-GFP fusion protein |
1 ml, 108 p.f.u./ml |
$499.00
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